http://hdl.handle.net/10174/204 2026-04-03T18:16:06Z 2026-04-03T18:16:06Z Cascais, João Lamas, Luís Monteiro, Susana http://hdl.handle.net/10174/37065 2024-06-25T10:28:59Z 2023-03-03T00:00:00Z

Title: Caracterização de claudicação com origem nas regiões metacarpiana e /ou metatarsiana proximais utilisando sistemas de análise objetiva de movimento Authors: Cascais, João; Lamas, Luís; Monteiro, Susana Abstract: Introdução e objetivos: A dressage e a Alta Escola são as disciplinas equestres nas quais os cavalos têm a maior taxa de ocorrência de desmite proximal do ligamento suspensor do boleto (LSB). A claudicação é uma grave consequência desta patologia. Considerando a importância da qualidade dos andamentos e como esta é influenciada por assimetrias, a análise objetiva de movimento pode contribuir para a quantificação e monitorização da locomoção. Este estudo retrospetivo teve como propósito investigar objetivamente se existia algum tipo de claudicação característico em cavalos com desmite proximal do LSB entre 2017 e 2022. Verificou, também, a correlação entre avaliações subjetiva e objetiva. Metodologia e resultados: Vinte e um cavalos Lusitanos foram incluídos neste estudo com base em registos de resposta positiva à anestesia diagnóstica da região proximal do LSB dos membros afetados. Dois sistemas de análise objetiva de movimento foram usados para a obtenção dos dados quantitativos das avaliações de todos os cavalos a trote à mão em linha reta no piso duro, assim como das avaliações em círculo nos pisos mole e duro. Claudicação de impacto foi a mais frequentemente encontrada em membros anteriores (MA), independentemente da superfície de avaliação. Por outro lado, os membros posteriores (MP) foram caracterizados por apresentarem claudicação de impulso ou de impacto e impulso. Relativamente à análise subjetiva, a maioria das claudicações eram de grau moderado, sendo que os MA foram identificados mais facilmente quando se encontravam no lado de fora dos círculos, já os MP aquando do lado de dentro dos mesmos. De entre as correlações verificadas, as únicas estatisticamente significativas foram entre o |MaxDiff| da cabeça e as classificações de grau de claudicação, quer os membros lesionados estivessem no interior (p = 0,02375) ou no exterior (p = 0,04167) do círculo na avaliação em piso mole. Principais conclusões: Apesar do LSB ser uma estrutura elástica, a sua ação e tensão principais ocorrem durante a fase de impacto da passada já que este previne a hiperextensão do boleto, o que explica que nos MA, devido a maiores cargas e falha na absorção do choque, este padrão de claudicação seja mais marcado do que nos MP. Quanto a estes últimos, a claudicação de impulso foi predominante e pode ser justificada pelo maior estiramento da parte proximal do LSB conforme o animal usa a potência propulsora e impulsora dessa estrutura dos MP durante esta fase da passada, em consequência de falha no armazenamento e uso eficaz da energia elástica na origem do(s) LSB lesionado(s). Os sistemas quantitativos usados neste estudo provaram ser úteis na caracterização de claudicação oriunda das regiões metacarpiana/tarsiana proximais em cavalos Lusitanos em treino e a competirem em dressage ou em exibições de Alta Escola. Este foi o primeiro estudo realizado no âmbito desta patologia músculo-esquelética com recurso a sistemas objetivos de locomoção, tendo como intuito aumentar a eficácia na prevenção e no diagnóstico precoce e direcionado através de adaptações dos pisos e regimes de trabalho, dos aprumos e da ferração, assim como dos tipos de claudicação apresentados.

2023-03-03T00:00:00Z Branco, Sandra http://hdl.handle.net/10174/37059 2024-06-25T10:28:21Z 2023-05-23T23:00:00Z

Title: O papel dos médicos veterinários no reconhecimento e resposta a maus-tratos a animais em contexto de violência doméstica e outras formas de violência Authors: Branco, Sandra Abstract: OS MÉDICOS VETERINÁRIOS TÊM PAPEL FUNDAMENTAL NO RECONHECIMENTO E RESPOSTA A MAUS-TRATOS A ANIMAIS EM CONTEXTO DE VIOLÊNCIA DOMÉSTICA. No campo da Patologia Forense têm capacidade para reconhecer lesões que podem conferir evidência de crueldade ou maus-tratos a animais (necrópsia). O patologista vai descrever exaustivamente as lesões identificadas na necrópsia.

2023-05-23T23:00:00Z Marques, M Nunes, J Fialho, Luisa Martins, Luís Burke, Anthony Souza, E Craveiro, Alexandre Costa, Ana Branco, Sandra Antunes, Célia http://hdl.handle.net/10174/32909 2022-12-27T17:19:58Z 2021-10-13T23:00:00Z

Title: Development of a canine skin analog Authors: Marques, M; Nunes, J; Fialho, Luisa; Martins, Luís; Burke, Anthony; Souza, E; Craveiro, Alexandre; Costa, Ana; Branco, Sandra; Antunes, Célia Abstract: Background. Skin covers an extensive area of the body and comprises three major layers, subcutis, dermis, and epidermis. This organ is one of the most tested for toxicity and safety evaluation during the process of drug research and development, in past usually performed in vivo using animals. On the last years a concern of sustainable and eco-friendly approach has been developing, with the expansion of non-animal alternatives (Klicks et al.; Mathes et al.).A histotypical cell cultured-derived tissue may be used to replace animal testing and are imperative to avoid armful, drawn-out tests to assess chemicals for their capacity to erode, bother or sensitize the skin. There are validated epidermal models for human (EPISKINTM and EpiDermTM) and rat (TER) skin (Flaten et al.; NIEHS; Netzlaff et al.). The aim of our study was the development of a histotypical canine skin equivalent, that can be used for the assessment of corrosion, irritation and sensibilization, avoiding in vivo animal testing. Methods. Canine keratinocyte progenitor cells were seeded in inserts and were allowed to grow until differentiation was reached, using an adapted version of the CELLnTEC commercial protocol, specific for human cells (CELLnTEC). For histological analysis, samples were fixed in 10% neutral-buffered formalin. Three-micrometer paraffin sections were routinely processed for biopsies and stained with hematoxylin and eosin. Corrosion, irritation and sensibilization protocols were adapted from human equivalent validated tests. Results. A multilayer (3-4 cell layers thick) of canine keratinocytes was developed in air-lift culture, originating a stratified epidermal-like tissue, confirmed by histological analysis. This epidermal-like tissue exhibited functional characteristics of normal epidermis. It showed adequate impermeabilization, after 0.1% Triton X-100 exposure for 4h and responded adequately to the positive (5% SDS and glacial acetic acid) and negative (PBS) controls used in “in vitro” corrosion and irritation assessment. Conclusions. As predicted, a canine skin analog was developed. This is a promising skin model for non-animal safety tests of veterinary pharmaceuticals or cosmetics, reducing in vivo testing, and can be commercialized as a service or a product.

2021-10-13T23:00:00Z Nunes, J Marques, M Sterghite, D Branco, S Martins, Luís Silva, M Campos-Gonçalves, I Souza, E Filho, César Craveiro, Alexandre Alpizar-Jara, Russel Burke, Anthony Costa, Ana Antunes, Célia http://hdl.handle.net/10174/32903 2022-12-27T17:03:18Z 2021-10-13T23:00:00Z

Title: Biocompatibility and biosafety analysis of chitosan hydrogels using organotypic epidermal models Authors: Nunes, J; Marques, M; Sterghite, D; Branco, S; Martins, Luís; Silva, M; Campos-Gonçalves, I; Souza, E; Filho, César; Craveiro, Alexandre; Alpizar-Jara, Russel; Burke, Anthony; Costa, Ana; Antunes, Célia Abstract: Background: Frequent dog consultations are due to skin wounds (Holland, 2019), with different trauma causes, like bites, burns and others. The majority of these wounds are not fatal, but cause long-lasting discomfort, demanding frequent medical care which may affect owners financially (Fahie & Shettko, 2007). Despite several pharmaceuticals available for dog wound treatment, new antibiotic-free efficient options, also more ecological are needed. Chitosan hydrogels offer a promising solution. Besides, being very ecological and biodegradable, chitosan hydrogels allow the maintenance of a moist environment that assists the exchange of fluids, essential for wound healing, and can also incorporate agents to avoid the development of infectious agents (Stashak, Farstvedt, & Othic, 2004). The aim of this study was to screen chitosan-based hydrogels for veterinary applications, supplied by the company BrInova Biochemistry as part of a collaborative project (NAQUIBIO DPSA). Screening was based on toxicity, biosafety, and efficacy “in vitro” tests, using organotypic epidermal models. Methods: Epidermal canine keratinocytes and human fibroblasts primary cells (supplied by CELLnTEC) were seeded in flasks and incubated to reach a desired level of growth, as indicated by the supplier. For toxicity evaluation, both cell lines were used. Viability tests were performed in 96 wells cell-culture plaques. Culture media and Triton X-100 was used as negative and positive controls, respectively. Firstly, hydrogels and potentially useful additives were tested individually, in several concentrations. Nontoxic components and doses were used in the production of several hydrogel-based composites, subsequentially tested. Viability was accessed by dehydrogenase activity (CCK-8, Sigma-Aldrich). For the efficacy of the hydrogel-based composites a wound scratch (WS) cellular assay was used, applied to both cell lines. For biosafety tests, a 3D keratinocyte culture was prepared, and irritation, corrosion, and sensitization protocols (adapted of EPISKINTM) were used. Results: Toxicity testes allowed the selection of 4 hydrogels, 2 nanoparticles and 2 plant essential oils to use in hydrogel-based composites, as well as the suitable concentrations range for each. Based on that information, 16 composites were prepared by other members of the NAQUIBIO team, and their toxicity was also evaluated, allowing the selection of 6 hydrogel-based composites. Those composites were evaluated for efficacy by a WS assay and the best compounds were those that could induce a fast closure of the scratch gap. The intersection of the toxicity and efficacy results allowed the selection of one hydrogel-based composite as the most promising one, whose biosafety was later evaluated. This proved to be non-corrosive, non-toxic and non-sensitizing. This compound was selected to proceed to the animal testing phase, in accordance with the goals of the NAQUIBIO project. Conclusions: As initially planned, we were able to select a mixture of hydrogels with essential oils and nanoparticles, to be further in vivo evaluated in dogs, or more specifically we have in advanced development a new wound dressing for veterinary use, capable of accelerating and creating better conditions for wound healing.

2021-10-13T23:00:00Z